WNK1 antibody (Middle Region)
-
- Target See all WNK1 Antibodies
- WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
-
Binding Specificity
- AA 1154-1195, Middle Region
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This WNK1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase WNK1(WNK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- DNPEEIATIM VNNDFILAIE RESFVDQVRE IIEKADEMLS ED
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
-
Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase WNK1(WNK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: WNK lysine deficient protein kinase 1
Protein Name: Serine/threonine-protein kinase WNK1 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human WNK1 (1154-1195aa DNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSED), different from the related mouse and rat sequences by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product WNK1 Primary Antibody
-
-
- Application Notes
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
- Alternative Name
- WNK1 (WNK1 Products)
- Synonyms
- HSAN2 antibody, HSN2 antibody, KDP antibody, PRKWNK1 antibody, PSK antibody, p65 antibody, WNK1 antibody, kdp antibody, psk antibody, hsn2 antibody, hsan2 antibody, pha2c antibody, prkwnk1 antibody, 6430573H23Rik antibody, EG406236 antibody, Hsn2 antibody, Prkwnk1 antibody, mKIAA0344 antibody, ATWNK1 antibody, T9J14.14 antibody, T9J14_14 antibody, ZIK4 antibody, with no lysine (K) kinase 1 antibody, WNK lysine deficient protein kinase 1 antibody, WNK lysine deficient protein kinase 1 L homeolog antibody, serine/threonine-protein kinase WNK1 antibody, with no lysine (K) kinase 1 antibody, WNK1 antibody, wnk1.L antibody, wnk1 antibody, LOC100056745 antibody, Wnk1 antibody
- Background
-
WNK lysine deficient protein kinase 1, also known as WNK1, is an enzyme that in humans is encoded by the WNK1 gene. The human gene is located on short arm of chromosome 12 (12p13.3). This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.
Synonyms: HSAN2 | HSN2 | KDP | Kinase deficient protein | p65 | PRKWNK1 | PSK | WNK1 | WNK 1 | Q9H4A3 - Gene ID
- 65125
- UniProt
- Q9H4A3
-