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SH3BP2 antibody (AA 432-481)

SH3BP2 Reactivity: Human, Mouse, Rat, Monkey, Cow WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6744458
  • Target See all SH3BP2 Antibodies
    SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))
    Binding Specificity
    • 7
    • 7
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 432-481
    Reactivity
    • 39
    • 12
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Monkey, Cow
    Host
    • 35
    • 4
    • 1
    Rabbit
    Clonality
    • 38
    • 2
    Polyclonal
    Conjugate
    • 21
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SH3BP2 antibody is un-conjugated
    Application
    • 27
    • 20
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human SH3BP2
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit, Guinea pig (92%) Bovine (84%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa432-481 of human SH3BP2 (P78314, NP_003014). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Opossum (100%), Elephant, Bat, Rabbit, Guinea pig (92%), Galago (85%), Bovine (84%), Zebra finch (83%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product SH3BP2 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))
    Alternative Name
    SH3BP2 (SH3BP2 Products)
    Synonyms
    SH3BP2 antibody, 3BP-2 antibody, 3BP2 antibody, CRBM antibody, CRPM antibody, SH3 domain binding protein 2 antibody, SH3-domain binding protein 2 antibody, SH3BP2 antibody, Sh3bp2 antibody
    Background
    Name/Gene ID: SH3BP2

    Synonyms: SH3BP2, Abl-SH3 binding protein 2, 3BP-2, 3BP2, Cherubism, CRPM, RES4-23, SH3 domain-binding protein 2, TNFAIP3 interacting protein 2, CRBM, SH3-domain binding protein 2
    Gene ID
    6452
    NCBI Accession
    NP_003014
    UniProt
    P78314
    Pathways
    TCR Signaling
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