SPAG16 antibody (AA 139-152)

Catalog No. ABIN490390

Product Details anti-SPAG16 Antibody

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Binding Specificity: AA 139-152

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPAG16 antibody is un-conjugated

Application: ELISA

Specificity: Human SPAG16. This antibody is expected to recognize isoform 1 and 2 (NP_078808.3, NP_001020607.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey (100%) Gibbon, Marmoset (93%) Panda, Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KGVTELRTVGNVPD from an internal region of human SPAG16 (NP_078808.3, NP_001020607.1). Percent identity by BLAST analysis: Human, Monkey (100%), Gibbon, Marmoset (93%), Panda, Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 30  kDa band in Human Testis lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 70.8  kDa according to NP_078808.3 and of 20.6  kDa according to NP_001020607.1. The 30  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SPAG16

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Alternative Name: SPAG16 (SPAG16 Products)

Synonyms: PF20 antibody, WDR29 antibody, 4921511D23Rik antibody, 4930524F24Rik antibody, 4930585K05Rik antibody, AV261009 antibody, Pf20 antibody, Wdr29 antibody, RGD1565062 antibody, sperm associated antigen 16 antibody, sperm-associated antigen 16 protein antibody, SPAG16 antibody, LOC694114 antibody, Spag16 antibody

Background: Name/Gene ID: SPAG16

Synonyms: SPAG16, Pf20 protein homolog, WDR29, PF20, Sperm associated antigen 16, WD repeat domain 29

Gene ID: 79582

NCBI Accession: NP_078808, NP_001020607

UniProt: Q8N0X2