V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (C-Term) antibody (Biotin)

Antigen: V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyms: ERBB3

Epitope: C-Term

Clonality: Polyclonal

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Western Blotting (WB)

Catalog no.: ABIN492350

Additional Information

Characteristics: Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

Alternative name: ERBB3 / HER3

Gene ID: 2065

Swiss-Prot: P21860

Immunogen: Synthetic petide conjugated to KLH. Epitope: C-terminus

Cross-Reactivity: Human

Isotype: IgG1

Description: ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsickinase activity due to an amino acid substitution within the ATP binding site. Uponheterodimerization with the other erbB family members, erbB3 becomes phosphorylated atmultiple tyrosine residues that represent docking sites for downstream signaling proteins,e. g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.

Specificity: This antibody specifically recognizes the C-terminus of erbB3 at 185 kDa. Species: Human. Others not tested. Add. Information: Mol. weight: 185 kDa.

Application Details

Protocol: Positive Control Provided. Cell lysate from untreated SW620Description: Cell lysate from untreated SW620 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from starved cells SW 620 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Application Notes: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW620 cells (See Protocols for moredetails). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Buffer: PBS, 0.09% Sodium Azide/PEG and Sucrose.Label: Biotin

Storage: Aliquote and freeze in liquid nitrogenAntibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.

Restrictions: For Research Use only