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Rhodopsin antibody

RHO Reactivity: Human, Cow, Rat, Mouse, Pig WB, IHC, ICC, IF Host: Mouse Monoclonal B630 unconjugated
Catalog No. ABIN4956272
  • Target See all Rhodopsin (RHO) Antibodies
    Rhodopsin (RHO)
    Reactivity
    • 39
    • 17
    • 16
    • 15
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Cow, Rat, Mouse, Pig
    Host
    • 39
    • 26
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 35
    • 33
    • 1
    Monoclonal
    Conjugate
    • 28
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Rhodopsin antibody is un-conjugated
    Application
    • 52
    • 42
    • 35
    • 25
    • 14
    • 12
    • 10
    • 8
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Purification
    affinity purified antibody
    Clone
    B630
    Isotype
    IgG1
    Top Product
    Discover our top product RHO Primary Antibody
  • Application Notes
    Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4°C short term or -20°C long term.
  • Target
    Rhodopsin (RHO)
    Alternative Name
    Rhodopsin (RHO Products)
    Synonyms
    CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody
    Target Type
    Chemical
    Background
    Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha, and a beta,gamma, subunit. The alpha, subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-B630 was raised against bovine outer segment discs and shows convincing staining for rhodopsin both on western blots and on sections of retina. The protein is concentrated in rod outer segments. The HGNC name for this protein is RHO.
    Pathways
    WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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