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ATG16L1 antibody (ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae)) (N-Term)

Details for Product anti-ATG16L1 Antibody No. ABIN497988, Supplier: Log in to see
Antigen
  • 1500009K01Rik
  • Apg16l
  • APG16L
  • atg16
  • ATG16A
  • atg16l
  • Atg16l
  • ATG16L
  • IBD10
  • Wdr30
  • WDR30
Epitope
N-Term
24
20
16
16
13
12
12
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
67
48
12
10
6
4
2
2
2
2
1
Host
Rabbit
133
17
14
2
Clonality
Polyclonal
Conjugate
This ATG16L1 antibody is un-conjugated
6
6
5
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
125
62
33
27
18
16
12
6
6
5
4
1
1
Supplier
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Immunogen ATG16 antibody was raised against a 18 amino acid peptide from near the amino terminus of Human ATG16 (Genbank accession No. NP_110430).
Isotype IgG
Specificity Recognizes ATG16 (N-term).
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Immunoaffinity Chromatography
Alternative Name APG16L / ATG16L1 (ATG16L1 Antibody Abstract)
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.Synonyms: APG16-like 1, Autophagy-related protein 16-1
Gene ID 55054
NCBI Accession NP_110430
UniProt Q676U5
Application Notes ELISA. Western blot: ATG16 antibody can be used for the detection of ATG16 at 0.5-1 μg/mL. Immunocytochemistry. Positive Control: HeLa Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-ATG16L1 antibody (ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae)) (N-Term) (ABIN497988) AP20063PU-N ATG16 antibody staining of HeLa Cell Lysate by Immunocytochemistry at 2 μ...
Immunofluorescence (IF) image for anti-ATG16L1 antibody (ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae)) (N-Term) (ABIN497988) AP20063PU-N ATG16 antibody staining of HeLa Cell Lysate by Immunofluorescence at 4.75...
Western Blotting (WB) image for anti-ATG16L1 antibody (ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae)) (N-Term) (ABIN497988) AP20063PU-N ATG16 antibody staining of HeLa Cell Lysate by Western Blotting at (A) 1 ...
Background publications Gozuacik, Kimchi: "Autophagy as a cell death and tumor suppressor mechanism." in: Oncogene, Vol. 23, Issue 16, pp. 2891-906, 2004

Mizushima, Kuma, Kobayashi, Yamamoto, Matsubae, Takao, Natsume, Ohsumi, Yoshimori: "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1679-88, 2003

Kamada, Funakoshi, Shintani, Nagano, Ohsumi, Ohsumi: "Tor-mediated induction of autophagy via an Apg1 protein kinase complex." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1507-13, 2000

Mizushima, Noda, Ohsumi: "Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway." in: The EMBO journal, Vol. 18, Issue 14, pp. 3888-96, 1999

Kisen, Tessitore, Costelli, Gordon, Schwarze, Baccino, Seglen: "Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells." in: Carcinogenesis, Vol. 14, Issue 12, pp. 2501-5, 1994