LARGE antibody (Internal Region)

Catalog No. ABIN498027

The Goat Polyclonal anti-LARGE antibody (ABIN498027) specifically detects LARGE in WB and EIA. The antibody is reactive with Human, Cow and Dog samples.

Quick Overview for LARGE antibody (Internal Region) (ABIN498027)

Target: LARGE (Like-Glycosyltransferase (LARGE))

Reactivity: Human, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This LARGE antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LARGE Antibody

Binding Specificity: AA 421-433, Internal Region

Sequence: C-SEADVNSENL QKQ

Specificity: Recognizes (421-433).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004728.1. Genename: LARGE

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/2000. Western blot: 1-3 μg/mL. Approx 75 kDa band observed in Human Kidney, and Heart lysates(calculated MW of 88.5 kDa according to NP_004728.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LARGE

Target: LARGE (Like-Glycosyltransferase (LARGE))

Alternative Name: LARGE

Background: Synonyms: Acetylglucosaminyltransferase-like 1A, Glycosyltransferase-like protein LARGE1, KIAA0609, LARGE, LARGE1

Gene ID: 9215

NCBI Accession: NP_004728

UniProt: O95461