NLRP2 antibody (C-Term)

Catalog No. ABIN498067

This Goat Polyclonal antibody specifically detects NLRP2 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for NLRP2 antibody (C-Term) (ABIN498067)

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NLRP2 Antibody

Binding Specificity: C-Term

Sequence: C-KNPQLIIDTE KHHP

Specificity: Recognizes NALP2 / PAN1

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus (near) of the protein sequence according to NP_060322.1. Genename: NLRP2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 140 kDa in lysates of cell line K562.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NLRP2

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Alternative Name: NALP2

Background: NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1beta-processing activity and is thought to function as a modulator of NF-kappaB and procaspase-1 activation in macrophages. It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.Synonyms: LRR and PYD domains-containing protein 2, NACHT, NBS1, NLRP2, PAN1, PYPAF2

Gene ID: 55655

NCBI Accession: NP_060322

UniProt: Q9NX02

Pathways: Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome