FOSB antibody

Catalog No. ABIN498127

Product Details anti-FOSB Antibody

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOSB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of FosB protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for FOSB

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Alternative Name: FOSB (FOSB Products)

Synonyms: FOSB antibody, zgc:92354 antibody, AP-1 antibody, G0S3 antibody, GOS3 antibody, GOSB antibody, fra-2 antibody, FBJ murine osteosarcoma viral oncogene homolog B antibody, FosB proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene B antibody, FOSB antibody, fosb antibody, Fosb antibody

Background: Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein II,a stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.Synonyms: G0/G1 switch regulatory protein 3, G0S3, GOS3, GOSB, Protein fosB

Molecular Weight: approx. 35 kDa

Gene ID: 2354

NCBI Accession: NP_006723

UniProt: P53539