KSR1 antibody

Catalog No. ABIN498178

Product Details anti-KSR1 Antibody

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of KSR protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Alternative Name: Kinase Suppressor of Ras 1 (KSR1 Products)

Synonyms: CG2899 antibody, Dmel\\CG2899 antibody, EK3-1 antibody, KSR antibody, KSR-1 antibody, Ksr antibody, Ksr-1 antibody, SR3-1 antibody, dKsr antibody, l(3)j5E2 antibody, AW492498 antibody, B-KSR1 antibody, D11Bhm183e antibody, D11Bhm184e antibody, RSU2 antibody, hb antibody, kinase suppressor of ras 1 antibody, kinase suppressor of ras antibody, KSR1 antibody, ksr antibody, Ksr1 antibody

Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 has intrinsic kinase activity towards serine/threonine residues and is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation.Synonyms: KSR, KSR-1, KSR1

Molecular Weight: approx. 115 kDa

Gene ID: 8844

NCBI Accession: NP_055053

UniProt: Q8IVT5