MBD3 antibody

Catalog No. ABIN498302

The Rabbit Polyclonal anti-MBD3 antibody is suitable to detect MBD3 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for MBD3 antibody (ABIN498302)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MBD3 Antibody

Specificity: This antibody detects endogenous levels of MBD3 protein. (region surrounding His250)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (Bhattacharya et al.). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (Hendrich et al.). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases (Petronzelli et al.). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (Bader et al.).Synonyms: Methyl-CpG-binding domain protein 3

Molecular Weight: approx. 33 kDa

Gene ID: 53615

NCBI Accession: NP_003917

UniProt: O95983

Pathways: Chromatin Binding