HK3 antibody

Catalog No. ABIN498424

This Rabbit Polyclonal antibody specifically detects HK3 in WB. It exhibits reactivity toward Human.

Quick Overview for HK3 antibody (ABIN498424)

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HK3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HK3 Antibody

Specificity: This antibody detects endogenous levels of HXK III protein. (region surrounding Val848)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HK3

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Alternative Name: Hexokinase-3

Background: Hexokinases phosphorylate glucose to produce glucose 6 phosphate, thus committing glucose to the glycolytic pathway. In vertebrates there are four major glucose phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Similar to hexokinases I and II, the allosteric enzyme hexokinase III is inhibited by its product glucose 6 phosphate. The catalytic activity of hexokinase III is associated with the C terminus while regulatory function is associated with the N terminus.Synonyms: HK3, HKIII, Hexokinase type III

Molecular Weight: approx. 99 kDa

Gene ID: 3101

NCBI Accession: NP_002106

UniProt: P52790

Pathways: Carbohydrate Homeostasis, Warburg Effect