PDGFA antibody

Catalog No. ABIN498533

Product Details anti-PDGFA Antibody

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This detects endogenous levels of PDGF-A protein. (region surrounding Lys154)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PDGFA

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: PDGFA (PDGFA Products)

Synonyms: PDGF-A antibody, PDGF1 antibody, pdgfa antibody, pdgfa-2 antibody, zgc:100777 antibody, pdgf antibody, pdgf1 antibody, pdgf-a antibody, AA409063 antibody, CD142 antibody, Cf-3 antibody, Cf3 antibody, TF antibody, PDGFACP antibody, wu:fc32c12 antibody, zgc:153249 antibody, platelet derived growth factor subunit A antibody, platelet-derived growth factor alpha polypeptide a antibody, coagulation factor III antibody, platelet derived growth factor, alpha antibody, platelet derived growth factor subunit A S homeolog antibody, platelet-derived growth factor alpha polypeptide b antibody, PDGFA antibody, pdgfaa antibody, pdgfa antibody, F3 antibody, Pdgfa antibody, pdgfa.S antibody, pdgfab antibody

Background: PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A

Molecular Weight: approx. 21 kDa

Gene ID: 5154

NCBI Accession: NP_002598

UniProt: P04085

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling