MTOR antibody

Catalog No. ABIN498610

This Rabbit Polyclonal antibody specifically detects MTOR in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MTOR antibody (ABIN498610)

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MTOR Antibody

Specificity: This antibody detects endogenous levels of mTOR protein. (region surrounding Ser2442)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: mTOR / FRAP1

Background: Regulatory associated protein of mTOR, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (mTOR), and is essential for mTOR signalling in vivo. Raptor binding to mTOR is critical for mTORcatalysed substrate phosphorylation of 4EBP1. The Raptor-mTOR complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of mTOR kinase activity under nutrient-deprived conditions and is an important component in the mTOR pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR

Molecular Weight: approx. 289 kDa

Gene ID: 2475

NCBI Accession: NP_004949

UniProt: P42345

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect