FOXO4 antibody (pSer197)

Catalog No. ABIN498704

The Rabbit Polyclonal anti-FOXO4 antibody is suitable to detect FOXO4 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for FOXO4 antibody (pSer197) (ABIN498704)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO4 Antibody

Binding Specificity: pSer197

Specificity: This antibody detects endogenous levels of AFX1 protein only when phosphorylated at Ser197.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: FOXO4 / AFX1 / MLLT7

Background: FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serineproline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 (in humans, this phosphorylation can occur at several sites, including Ser 197) and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2,13) and t(1,3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x,11) translocation of the AFX1 gene, which involves the fusion of a serine-prolinerich sequence of AFX1 to the carboxyterminus of a truncated MLL, results in acute lymphocytic leukemia.Synonyms: Fork head domain transcription factor AFX1, Forkhead box protein O4

Molecular Weight: approx. 55, 70 kDa

Gene ID: 4303

NCBI Accession: NP_005929

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation