CNOT4 antibody

Catalog No. ABIN499042

Product Details anti-CNOT4 Antibody

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of CNOT4 protein. (region surrounding Glu95)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CNOT4

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Alternative Name: CNOT4 (CNOT4 Products)

Synonyms: CLONE243 antibody, NOT4 antibody, NOT4H antibody, Not4 antibody, Not4h antibody, Not4hp antibody, CNOT4 antibody, CCR4-NOT transcription complex subunit 4 antibody, RNA binding (RRM/RBD/RNP motifs) family protein antibody, CCR4-NOT transcription complex, subunit 4 antibody, CCR4-NOT transcription complex subunit 4 L homeolog antibody, CCR4-NOT transcription complex, subunit 4b antibody, CNOT4 antibody, AT3G45630 antibody, Cnot4 antibody, cnot4.L antibody, cnot4b antibody

Background: CNOT4 (CCR4 NOT transcription complex subunit 4) has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.Synonyms: CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, NOT4, NOT4Hp, Potential transcriptional repressor NOT4Hp

Molecular Weight: approx. 52 kDa

Gene ID: 4850

NCBI Accession: NP_001008226

UniProt: O95628