STAT4 antibody (Internal Region)

Catalog No. ABIN499125

The Goat Polyclonal anti-STAT4 antibody (ABIN499125) specifically detects STAT4 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for STAT4 antibody (Internal Region) (ABIN499125)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-STAT4 Antibody

Binding Specificity: AA 369-383, Internal Region

Sequence: TLSNRRFVLC GTNVK

Specificity: Recognizes STAT4 (aa 369-383).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (aa 369-383) of the protein sequence according to Human STAT4 (NP_003142.1). Genename: STAT4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.5-1.0 μg/mL. This antibody detects a band of approx. 85 kDa in transfected HEK293transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: STAT4

Background: The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4

Gene ID: 6775

NCBI Accession: NP_003142

UniProt: Q14765

Pathways: JAK-STAT Signaling