UCHL1 antibody (Internal Region)

Catalog No. ABIN499139

The Goat Polyclonal anti-UCHL1 antibody is suitable to detect UCHL1 in samples from Human, Rat, Cow, Pig and Dog. It has been validated for WB and EIA.

Quick Overview for UCHL1 antibody (Internal Region) (ABIN499139)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UCHL1 Antibody

Binding Specificity: AA 58-68, Internal Region

Sequence: C-QHENFRKKQI E

Specificity: Recognizes UCHL1 (aa 58-68).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence (aa 58-68) from the internal region of the protein sequence according to NP_004172.2. Genename: UCHL1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 26 kDa in Human Brain(Cerebellum and Hippocampus) and Rat Brain lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1 / PGP9.5

Background: Protein gene product 9.5 (PGP9.5) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.Synonyms: Neuron cytoplasmic protein 9.5, PGP 9.5, UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1

Molecular Weight: 24.8kDa (Human NP_004172.2, Mouse NP_035800.2 and Rat NP_058933.2)

Gene ID: 7345

NCBI Accession: NP_004172

UniProt: P09936

Pathways: Feeding Behaviour