SIGLEC6 antibody (Internal Region)

Catalog No. ABIN499145

Product Details anti-SIGLEC6 Antibody

Target: SIGLEC6 (Sialic Acid Binding Ig-Like Lectin 6 (SIGLEC6))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIGLEC6 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-KWMKYGYTSS K

Specificity: This antibody is expected to recognize all reported isoforms (NP_001236.4, NP_942142.3, NP_942143.3).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001236.4, NP_942142.3 and NP_942143.3. Genename: SIGLEC6

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.5-1.5 μg/mL. This antibody detects bands at approx. 60 kDa and 30 kDa in HumanPlacenta lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SIGLEC6

Target: SIGLEC6 (Sialic Acid Binding Ig-Like Lectin 6 (SIGLEC6))

Alternative Name: CD327 / SIGLEC6 (SIGLEC6 Products)

Synonyms: SIGLEC6 antibody, CD327 antibody, CD33L antibody, CD33L1 antibody, CD33L2 antibody, CDW327 antibody, OBBP1 antibody, sialic acid binding Ig like lectin 6 antibody, SIGLEC6 antibody

Background: SIGLEC6 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells by binding to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC6, which interacts with LEP, is expressed at high levels in placenta (cyto-and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine. It contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The gene for SIGLEC6 belongs to the immunoglobulin superfamily.Synonyms: CD33 antigen-like 1, CD33L, CD33L1, CDw327, OB-BP1, OBBP1, Obesity-binding protein 1, Sialic acid-binding Ig-like lectin 6, Siglec-6

Gene ID: 946

NCBI Accession: NP_001236

UniProt: O43699