PSMA4 antibody (Internal Region)

Catalog No. ABIN499149

Product Details anti-PSMA4 Antibody

Target: PSMA4 (Proteasome Subunit alpha 4 (PSMA4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMA4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-SDANVLTNEL R

Specificity: This antibody is expected to recognize both reported isoforms (NP_002780.1 and NP_001096138.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002780.1 and NP_001096138.1 Genename: PSMA4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 28 kDa in lysates of cell line K562and in lysates of Human Tonsil and Human Peripheral Blood Mononucleocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PSMA4

Target: PSMA4 (Proteasome Subunit alpha 4 (PSMA4))

Alternative Name: PSMA4 (PSMA4 Products)

Synonyms: wu:fe05d10 antibody, zgc:56176 antibody, DDBDRAFT_0204055 antibody, DDBDRAFT_0214953 antibody, DDB_0204055 antibody, DDB_0214953 antibody, C9 antibody, HC9 antibody, HsT17706 antibody, PSC9 antibody, proteasome subunit alpha 4 antibody, proteasome subunit alpha 4 S homeolog antibody, proteasome subunit alpha type 4 antibody, 20S proteasome subunit alpha-4 antibody, proteasome (prosome, macropain) subunit, alpha type 4 antibody, psma4 antibody, psma4.S antibody, PSMA4 antibody, CNF01860 antibody, psmA4 antibody, Psma4 antibody

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA4 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: HC9, Macropain subunit C9, Multicatalytic endopeptidase complex subunit C9, PSC9, Proteasome component C9, Proteasome subunit L, Proteasome subunit alpha type-4

Molecular Weight: 29.5kDa (NP_002780.1)

Gene ID: 5685

NCBI Accession: NP_001096137

UniProt: P25789

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA