SMAD Family Member 9 (SMAD9) (Internal Region) antibody

Details for Product No. ABIN499162
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Antigen
Synonyms SMAD8, MADH6, MADH9, PPH2, SMAD8A, SMAD8B, Madh8, Madh9, Smad8
Epitope
Internal Region
(10), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(48), (18), (17), (15), (12), (12), (12)
Host
Goat
(35), (7), (5), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(36), (20), (10), (10), (7), (6), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN499162
357.50 $
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Immunogen Peptide from the internal region of the protein sequence according to NP_005896.1
Sequence C-HSEPLMPHNA TYPD
Specificity This antibody recognizes SMAD9 and is not expected to cross-react with SMAD1 and SMAD5. Species Reactivity: Tested: Human Expected from sequence similarity: Mouse, Rat, Canine (Dog).
Purification Ammonium Sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Alternative Name SMAD9
Background SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.
Alternate names: MAD homolog 9, MADH9, Mothers against DPP homolog 9, Mothers against decapentaplegic homolog 9, SMAD 9, SMAD family member 9, SMAD-9
Gene ID 4093
NCBI Accession NP_001120689
UniProt O15198
Research Area Cancer, Signaling, Transcription Factors
Application Notes Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-1.0 µg/ml, approx 50kDa band observed in Human Skin lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH~7.3 0.02% Sodium Azide 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

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