Aconitase 1 antibody (Internal Region)

Catalog No. ABIN499173

Product Details anti-Aconitase 1 Antibody

Target: Aconitase 1 (ACO1)

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-QVDFNRRADS LQKNQ

Specificity: This antibody detects ACO1 / IREB1 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_002188.1 Genename: ACO1

Application Details

Application Notes: Peptide ELISA: 1: 8000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in Human Liver lysates(calculated MW of 98.0 kDa according to NP_002188.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1 / IREB1 (ACO1 Products)

Synonyms: ACONS antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, IRP1 antibody, Acon1 antibody, Ratireb antibody, ratireb antibody, Aco1 antibody, AI256519 antibody, Aco-1 antibody, Irebp antibody, Irp1 antibody, aconitase 1 antibody, aconitase 1, soluble antibody, aconitase 1 L homeolog antibody, ACO1 antibody, Aco1 antibody, aco1.L antibody

Background: Aconitase 1 (ACO1) also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IRES). IREs are translational regulatory sequences found in the 5'-UTR of ferritin, and in the 3'-UTR of transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis by regulating ferritin mRNA translation and TFRC mRNA stability.Synonyms: Aconitase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRP1, Iron regulatory protein 1, Iron-responsive element-binding protein 1

Gene ID: 48

NCBI Accession: NP_002188

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis