LIG1 antibody (Internal Region)

Catalog No. ABIN499175

The Goat Polyclonal anti-LIG1 antibody is suitable to detect LIG1 in samples from Human and Rat. It has been validated for WB.

Quick Overview for LIG1 antibody (Internal Region) (ABIN499175)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIG1 Antibody

Binding Specificity: Internal Region

Sequence: C-RVREDKQPEQ ATTS

Specificity: This antibody detects DNA ligase 1 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):RatSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1

Application Details

Application Notes: Peptide ELISA: 1: 16000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 125 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 105 kDa according to NP_000225.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA Ligase 1

Background: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.Synonyms: DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1

Gene ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA