BIM antibody (Intermediate Domain 2)

Catalog No. ABIN499469

The Rabbit Polyclonal anti-BIM antibody is suitable to detect BIM in samples from Human, Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for BIM antibody (Intermediate Domain 2) (ABIN499469)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-BIM Antibody

Binding Specificity: Intermediate Domain 2

Specificity: Bim antibody was raised against a peptide corresponding to amino acids near the center of Bim.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Bim (Intermediate Domain 2) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Bim antibody can be used for detection of Bim at 2.5 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

Gene ID: 10018

NCBI Accession: NP_619527

UniProt: O43521

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity