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CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (N-Term)

Details for Product anti-CFLAR Antibody No. ABIN499846, Supplier: Log in to see
Antigen
  • 2310024N18Rik
  • A430105C05Rik
  • c-Flip
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • CASH
  • Cash
  • CASP8AP1
  • Casper
  • cflar
  • CFLAR
  • CLARP
  • Clarp1
  • ENSMUSG00000072980
  • FLAME
  • FLAME-1
  • FLAME1
  • Flip
  • FLIP
  • Gm9845
  • I-FLICE
  • MRIT
Alternatives
anti-Human CFLAR antibody for Immunofluorescence
Epitope
N-Term
37
21
18
13
13
13
10
8
7
6
6
6
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
199
78
56
2
2
2
2
2
1
Host
Rabbit
155
60
4
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
7
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
179
73
48
36
34
22
22
15
13
7
3
3
1
1
1
Supplier
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Immunogen Bacteria FLIP (N-Terminus) Peptide
Isotype IgG
Specificity FLIP antibody was raised against a peptide corresponding to amino acids near the amino terminus of human FLIP The sequence is identical in all FLIP splice variants. FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
Purification Affinity chromatography purified via peptide column
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
UniProt O15519
Pathways Apoptosis
Application Notes ELISA. Western Blot: 1/500 to 1/1000. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (N-Term) (ABIN499846) Immunocytochemistry of FLIP in HeLa cells with AP30341PU-N FLIP antibody at 5 μg/ml.
Western Blotting (WB) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (N-Term) (ABIN499846) Western blot analysis of FLIP in K562 cell lysate with AP30341PU-N FLIP antibody at (...
Background publications Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997

Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997