NDUFAF2 antibody (C-Term)

Catalog No. ABIN500282

Product Details anti-NDUFAF2 Antibody

Target: NDUFAF2 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, Assembly Factor 2 (NDUFAF2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide affinity chromatography

Immunogen: Mimitin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Mimitin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for NDUFAF2

Target: NDUFAF2 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, Assembly Factor 2 (NDUFAF2))

Alternative Name: Mimitin (NDUFAF2 Products)

Synonyms: B17.2L antibody, MMTN antibody, NDUFA12L antibody, mimitin antibody, Ndufa12l antibody, RGD1560158 antibody, NDUFAF2 antibody, ndufa12l antibody, zgc:101036 antibody, 1810058I14Rik antibody, C86051 antibody, NADH:ubiquinone oxidoreductase complex assembly factor 2 antibody, NADH:ubiquinone oxidoreductase complex assembly factor 2 L homeolog antibody, NADH dehydrogenase (ubiquinone) complex I, assembly factor 2 antibody, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2 antibody, NDUFAF2 antibody, Ndufaf2 antibody, ndufaf2.L antibody, ndufaf2 antibody

Background: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80 % of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.Synonyms: B17.2-like, Myc-induced mitochondrial protein, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, NDUFA12L, NDUFAF2

Gene ID: 91942

NCBI Accession: NP_777549

UniProt: Q8N183

Pathways: SARS-CoV-2 Protein Interactome