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Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) (N-Term) antibody

Details for Product No. ABIN500363, Supplier: Login to see
Antigen
  • ACUG
  • BLAU
  • CARD15
  • CD
  • CLR16.3
  • IBD1
  • NLRC2
  • NOD2B
  • PSORAS1
  • Card15
  • F830032C23Rik
  • Nlrc2
Epitope
N-Term
25
14
12
8
5
3
2
2
1
Reactivity
Human
108
31
17
1
Host
Rabbit
76
16
16
7
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
97
37
24
17
17
14
11
7
3
3
2
2
1
Supplier
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Immunogen Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.
Isotype IgG
Specificity This antibody detects CARD15 / NOD2 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):ELISA.Western blot: 2 to 4 μg/mL.Immunohistochemistry on paraffin sections.
Purification Peptide affinity chromatography
Alternative Name CARD15 / NOD2 (NOD2 Antibody Abstract)
Background Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease (3,4) that is a main type of chronic inflammatory bowel disease.Synonyms: Caspase recruitment domain-containing protein 15, IBD1, Inflammatory bowel disease protein 1, Nucleotide-binding oligomerization domain-containing protein 2
Gene ID 64127
UniProt Q9HC29
Research Area Signaling, Transcription Factors
Pathways
Application Notes ELISA. Western blot: 2 to 4 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) (N-Term) antibody (ABIN500363) Immunocytochemistry of NOD2 in Jurkat cells with this product at 5 μg/ml.
Western Blotting (WB) image for anti-Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) (N-Term) antibody (ABIN500363) Western blot analysis of NOD2 in Jurkat cell lysate with this product at (A) 1, (B) 2...
Background publications Ogura, Bonen, Inohara et al.: "A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 603-6, 2001 (PubMed).

Hugot, Chamaillard, Zouali et al.: "Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 599-603, 2001 (PubMed).

Ogura, Inohara, Benito et al.: "Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4812-8, 2001 (PubMed).

Inohara, Koseki, del Peso et al.: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999 (PubMed).