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NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term)

Details for Product anti-NOD2 Antibody No. ABIN500365, Supplier: Log in to see
Antigen
  • ACUG
  • BLAU
  • Card15
  • CARD15
  • CD
  • CLR16.3
  • F830032C23Rik
  • IBD1
  • Nlrc2
  • NLRC2
  • NOD2B
  • PSORAS1
Epitope
C-Term
32
27
14
13
8
5
4
3
1
1
Reactivity
Human
136
50
17
1
Host
Rabbit
87
34
15
7
Clonality
Polyclonal
Conjugate
This NOD2 antibody is un-conjugated
5
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
126
49
44
39
37
34
26
11
4
3
3
2
1
Supplier
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Immunogen Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2.
Isotype IgG
Specificity This antiobody detects CARD15 / NOD2 at C-term. It has no cross-reaction with NOD1.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Peptide affinity chromatography
Alternative Name CARD15 / NOD2 (NOD2 Antibody Abstract)
Background Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease (3,4) that is a main type of chronic inflammatory bowel disease.Synonyms: Caspase recruitment domain-containing protein 15, IBD1, Inflammatory bowel disease protein 1, Nucleotide-binding oligomerization domain-containing protein 2
Gene ID 64127
UniProt Q9HC29
Research Area Signaling, Transcription Factors
Application Notes ELISA. Western blot: 2 - 4 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term) (ABIN500365) Immunocytochemistry of NOD2 in HeLa cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (C-Term) (ABIN500365) Western blot analysis of NOD2 in HeLa cell lysate with this product at (A) 2 and (B) ...
Background publications Ogura, Inohara, Benito, Chen, Yamaoka, Nunez: "Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4812-8, 2001 (PubMed).

Hugot, Chamaillard, Zouali, Lesage, Cézard, Belaiche, Almer, Tysk, OMorain, Gassull, Binder, Finkel, Cortot, Modigliani, Laurent-Puig, Gower-Rousseau, Macry, Colombel, Sahbatou, Thomas: "Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 599-603, 2001 (PubMed).

Ogura, Bonen, Inohara, Nicolae, Chen, Ramos, Britton, Moran, Karaliuskas, Duerr, Achkar, Brant, Bayless, Kirschner, Hanauer, Nuñez, Cho: "A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 603-6, 2001 (PubMed).

Inohara, Koseki, del Peso, Hu, Yee, Chen, Carrio, Merino, Liu, Ni, Núñez: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999 (PubMed).