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MTA2 antibody (Metastasis Associated 1 Family, Member 2) (AA 652-668)

Details for Product anti-MTA2 Antibody No. ABIN500495, Supplier: Log in to see
Antigen
  • pid
  • mta1l1
  • MTA2
  • DKFZp470E0911
  • MTA1L1
  • PID
  • fd61b05
  • wu:fd61b05
  • wu:fi29g02
  • zgc:63487
  • AW550797
  • Mata1l1
  • Mta1l1
  • mmta2
  • metastasis associated 1 family, member 2
  • metastasis-associated gene family, member 2
  • preimplantation development
  • mta2
  • MTA2
  • Mta2
  • pid
Alternatives
anti-Human MTA2 antibody for Western Blotting
Epitope
AA 652-668
15
13
6
5
5
3
3
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
106
62
55
18
14
11
8
7
6
5
4
4
3
3
3
2
2
1
Host
Rabbit
103
5
2
Clonality
Polyclonal
Conjugate
This MTA2 antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
94
23
20
19
13
12
7
4
3
2
1
1
1
1
Options
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Immunogen PID antibody was raised against a synthetic peptide corresponding to amino acids 652 to 668 of human PID (6), which differ from the mouse sequence by one amino acid (7).
Isotype IgG
Specificity This antibody detects MTA2.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification DEAE purified
Alternative Name MTA2 (MTA2 Antibody Abstract)
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex (6-8). PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6). PID/MTA2 is ubiquitously expressed in human tissues (8).Synonyms: MTA1-L1, MTA1L1, Metastasis-associated 1-like 1, Metastasis-associated protein MTA2, PID, p53 target protein in deacetylase complex
Gene ID 9219
UniProt O94776
Research Area Chromatin and Nuclear Signaling
Pathways Chromatin Binding
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, HeLa cell lysate can be used as positive control and a 75 kDaband can be detected. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-MTA2 antibody (Metastasis Associated 1 Family, Member 2) (AA 652-668) (ABIN500495) Immunocytochemistry staining of HeLa using AP30669PU-N at 10 μg/ml.
Western Blotting (WB) image for anti-MTA2 antibody (Metastasis Associated 1 Family, Member 2) (AA 652-668) (ABIN500495) Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A)...
Background publications Tanaka, Arakawa, Yamaguchi, Shiraishi, Fukuda, Matsui, Takei, Nakamura: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa, Nemoto, Shibue, Yamashita, Tokino, Taniguchi, Tanaka: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Oda, Arakawa, Tanaka, Matsuda, Tanikawa, Mori, Nishimori, Tamai, Tokino, Nakamura, Taya: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).