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RAPTOR (C-Term) antibody

Details for Product No. ABIN500571, Supplier: Log in to see
Antigen
Epitope
C-Term
16
13
13
11
10
7
2
2
2
1
1
Reactivity
Mouse (Murine)
94
50
28
1
Host
Rabbit
102
3
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
5
5
5
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
64
34
22
14
13
13
13
7
6
3
3
1
Supplier
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Immunogen Raptor (CT) polyclonal antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of mouse Raptor.
Isotype IgG
Cross-Reactivity (Details) Species reactivity (tested):Mouse
Purification Peptide affinity chromatography
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif, mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).Synonyms: KIAA1303, P150 target of rapamycin TOR-scaffold protein, RPTOR, Regulatory-associated protein of mTOR
UniProt Q8K4Q0
Application Notes ELISA. Western blot: 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-RAPTOR (C-Term) antibody (ABIN500571) Immunocytochemistry of Raptor in L1210 cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-RAPTOR (C-Term) antibody (ABIN500571) Western blot analysis of Raptor in L1210 cell lysate with this product at (A) 2 and (...
Background publications Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Yonezawa, Tokunaga, Oshiro, Yoshino: "Raptor, a binding partner of target of rapamycin." in: Biochemical and biophysical research communications, Vol. 313, Issue 2, pp. 437-41, 2003 (PubMed).

Hara, Yonezawa, Weng, Kozlowski, Belham, Avruch: "Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14484-94, 1998 (PubMed).