You are viewing an incomplete version of our website. Please click to reload the website as full version.

TNFSF11 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 11) (Center)

Details for Product anti-TNFSF11 Antibody No. ABIN500805, Supplier: Log in to see
Antigen
  • TNFSF11
  • BTF3L1
  • HUMBTFB
  • OCIF
  • OPG
  • TNFRSF11B
  • CD254
  • ODF
  • OPGL
  • OPTB2
  • RANKL
  • TRANCE
  • hRANKL2
  • sOdf
  • Ly109l
  • Trance
  • Opg
Alternatives
anti-Human TNFSF11 antibody for Enzyme Immunoassay
Epitope
Center
15
14
13
13
11
10
9
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
169
116
39
1
1
1
1
Host
Rabbit
163
47
29
7
1
Clonality
Polyclonal
Conjugate
This TNFSF11 antibody is un-conjugated
26
12
6
6
5
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
173
107
46
44
28
22
21
12
10
10
9
5
5
3
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen sRANK-L antibody was raised against a 14 amino acid peptide from near the center of Human sRANK-L.
Isotype IgG
Specificity This antibody detects CD254 / RANKL. It will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Immunoaffinity Chromatography
Alternative Name CD254 / RANKL (TNFSF11 Antibody Abstract)
Background The receptor activator of NF-kappaB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
Gene ID 8600
NCBI Accession NP_003692
Pathways NF-kappaB Signaling
Application Notes ELISA. Western blot: 0.25 - 0.5 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TNFSF11 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 11) (Center) (ABIN500805) Immunohistochemistry of sRANK-L in human liver tissue with this product at 5 μg/ml.
Western Blotting (WB) image for anti-TNFSF11 antibody (Tumor Necrosis Factor (Ligand) Superfamily, Member 11) (Center) (ABIN500805) Western blot analysis of sRANK-L in rat liver tissue lysate with this product at (A) ...
Background publications Bharti, Takada, Shishodia et al.: "Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6065-76, 2004 (PubMed).

Weitzmann, Cenci, Rifas et al.: "Interleukin-7 stimulates osteoclast formation by up-regulating the T-cell production of soluble osteoclastogenic cytokines." in: Blood, Vol. 96, Issue 5, pp. 1873-8, 2000 (PubMed).

Kong, Yoshida, Sarosi et al.: "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis." in: Nature, Vol. 397, Issue 6717, pp. 315-23, 1999 (PubMed).

Wong, Rho, Arron et al.: "TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25190-4, 1997 (PubMed).