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TSC2 antibody (Tuberous Sclerosis 2) (N-Term)

Details for Product anti-TSC2 Antibody No. ABIN501075, Supplier: Login to see
Antigen
  • TSC2
  • fc16b02
  • tuberin
  • wu:fc16b02
  • LAM
  • TSC4
  • Nafld
  • Tcs2
  • Rc
Alternatives
anti-Human TSC2 antibody for ELISA
Epitope
N-Term
42
40
27
27
26
24
24
23
23
22
21
20
20
20
20
20
20
20
20
20
18
17
16
16
15
15
13
13
13
13
11
8
8
6
6
6
6
6
6
6
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
518
376
210
6
Host
Rabbit
692
24
Clonality
Polyclonal
Conjugate
This TSC2 antibody is un-conjugated
51
51
51
42
42
42
12
9
9
9
9
9
9
9
9
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
387
291
221
137
104
66
52
14
8
6
4
4
1
Supplier
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Immunogen TSC2 (NT) was raised against a 14 amino acid peptide from near the amino terminus of human TSC2.
Isotype IgG
Specificity This antibody detects TSC2 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name Tuberin / TSC2 (TSC2 Antibody Abstract)
Background Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). At least three isoforms of TSC2 exist.Synonyms: TSC4, Tuberous sclerosis 2 protein
Gene ID 7249
NCBI Accession NP_000539
Pathways RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-TSC2 antibody (Tuberous Sclerosis 2) (N-Term) (ABIN501075) Immunocytochemistry of TSC2 in L1210 cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-TSC2 antibody (Tuberous Sclerosis 2) (N-Term) (ABIN501075) Western blot analysis of TSC2 in L1210 cell lysate with this product at (A) 2 and (B)...
Background publications Inoki, Ouyang, Li et al.: "Signaling by target of rapamycin proteins in cell growth control." in: Microbiology and molecular biology reviews : MMBR, Vol. 69, Issue 1, pp. 79-100, 2005 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Inoki, Li, Xu et al.: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003 (PubMed).

Tabancay, Gau, Machado et al.: "Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39921-30, 2003 (PubMed).