POLR3H antibody (Middle Region)

Catalog No. ABIN502958

The Rabbit Polyclonal anti-POLR3H antibody has been validated for WB. It is suitable to detect POLR3H in samples from Human, Mouse, Rat, Cow, Dog, Rabbit and Horse. There is 1 publication available.

Quick Overview for POLR3H antibody (Middle Region) (ABIN502958)

Target: POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3H Antibody

Binding Specificity: Middle Region

Sequence: AHDLYMDTGE EIRFRVVDES FVDTSPTGPS SADATTSSEE LPKKEAPYTL

Predicted Reactivity: Cow: 79%, Dog: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against POLR3H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR3H

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 175 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-POLR3H antibody (ABIN502958)

Collins, Wright, Edwards, Davis, Grinham, Cole, Goward, Aguado, Mallya, Mokrab, Huckle, Beare, Dunham: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, (2004) (PubMed).

Target Details for POLR3H

Target: POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))

Alternative Name: POLR3H

Background: POLR3H belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3H is a specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.
Alias Symbols: KIAA1665, MGC111097, MGC29654, RPC8, RPC22.9
Protein Interaction Partner: UBC, POLR3D, POLR3B, POLR1D, CRCP, POLR3A, POLR3C, HMG20B, POLR1C, POLR2H, POLR2E, POLR3E, CDC42EP4, POLR3G, RAE1, YWHAE, PCBP2, HSPA5,
Protein Size: 175

Molecular Weight: 20 kDa

Gene ID: 171568

NCBI Accession: NM_001018052, NP_001018062

UniProt: B0QYH9