POLR3H antibody (Middle Region)

Catalog No. ABIN2783401

There is 1 publication for this product available. The Rabbit Polyclonal anti-POLR3H antibody is suitable to detect POLR3H in samples from Human, Mouse, Rat, Cow, Dog, Rabbit and Horse. It has been validated for WB.

Quick Overview for POLR3H antibody (Middle Region) (ABIN2783401)

Target: POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3H Antibody

Binding Specificity: Middle Region

Purpose: POLR3H Antibody - middle region

Sequence: AHDLYMDTGE EIRFRVVDES FVDTSPTGPS SADATTSSEE LPKKEAPYTL

Predicted Reactivity: Cow: 79%, Dog: 86%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against POLR3H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR3H

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48519-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-POLR3H antibody (ABIN2783401)

Collins, Wright, Edwards, Davis, Grinham, Cole, Goward, Aguado, Mallya, Mokrab, Huckle, Beare, Dunham: "A genome annotation-driven approach to cloning the human ORFeome." in: Genome biology, Vol. 5, Issue 10, pp. R84, (2004) (PubMed).

Target Details for POLR3H

Target: POLR3H (Polymerase (RNA) III (DNA Directed) Polypeptide H (22.9kD) (POLR3H))

Alternative Name: POLR3H

Background:

Targeted Subunit: RPC8

Background Information: POLR3H belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3H is a specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.

Gene Name: Polymerase (RNA) III (DNA directed) polypeptide H (22.9kD)

Alternative Symbols: C25, RPC8, RPC22.9

Protein Name: DNA-directed RNA polymerase III subunit RPC8

Molecular Weight: 20kDa

Gene ID: 171568

NCBI Accession: NP_001018062

UniProt: B0QYH9