Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F) (N-Term) antibody

Details for Product No. ABIN502973
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Antigen
Synonyms 2810411G20Rik, 3010019O03Rik, 3110032A07Rik, RPC39, RPC6, zgc:56299, zgc:76983
Epitope
N-Term
(12), (3), (2), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Human
(25), (17), (17), (1), (1), (1), (1)
Host
Rabbit
(23), (2)
Clonality
Polyclonal
Application
Western Blotting (WB)
(25), (9), (6), (4), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502973
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human POLR3F
Sequence MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR
Predicted Reactivity Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Dog : 90 %
Characteristics This is a rabbit polyclonal antibody against POLR3F. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name POLR3F
Background POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.
Molecular Weight 36 kDa
Gene ID 10621
NCBI Accession NP_006457, NM_006466
UniProt Q9H1D9
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 316 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F) (N-Term) antibody WB Suggested Anti-POLR3F Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: PANC1 cell lysate
Background publications Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Validation Images
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