NEK9 antibody (Middle Region)

Catalog No. ABIN503005

This Rabbit Polyclonal antibody specifically detects NEK9 in WB. It exhibits reactivity toward Human, Mouse, Rat, Horse, Rabbit, Cow, Dog and Guinea Pig.

Quick Overview for NEK9 antibody (Middle Region) (ABIN503005)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NEK9 Antibody

Binding Specificity: Middle Region

Sequence: GGGGGGEEED SQQESETPDP SGGFRGTMEA DRGMEGLISP TEAMGNSNGA

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NEK9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NEK9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 979 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Alternative Name: NEK9

Background: NEK9 is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. NEK9 phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. NEK9 phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
Alias Symbols: DKFZp434D0935, MGC138306, MGC16714, NERCC, NERCC1, Nek8
Protein Interaction Partner: HUWE1, MDM2, UBC, SPDL1, REPIN1, CAP1, MCM5, NEK6, HSP90AA1, Nek9, Dynll1, MYC, SSRP1, NEK7, GABARAPL1, GABARAPL2, NSMAF, CHEK1, SUPT16H, BICD2, RAN, MBP, cdk1-a,
Protein Size: 979

Molecular Weight: 107 kDa

Gene ID: 91754

NCBI Accession: NM_033116, NP_149107

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection