Retinoblastoma Binding Protein 8 (RBBP8) antibody

Antigen: Retinoblastoma Binding Protein 8 (RBBP8)

Synonyms: RIM, CTIP, CtIP, 9930104E21Rik, RGD1308872, MGC80845, RBBP8, MGC139117, DKFZp469K0714

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN503204

Additional Information

Alternative name: RBBP8

Gene ID: RBBP8

UniProt: A6NKN2

Immunogen: The immunogen for anti-RBBP8 antibody: synthetic peptide directed towards the C terminal of human RBBP8

Sequence: ETVDMDCTLVSETVLLKMKKQEQKGEKSSNEERKMNDSLE DMFDRTTHEE

Cross-Reactivity: Dog (Canine), Human

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-RBBP8 antibody concentration is 1 mg/ml.

Description: RBBP8 is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

Characteristics: Antigen Size: 867 amino acids

Molecular Weight: 98kDa

Application Details

Application Notes: WB Suggested Anti-RBBP8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-RBBP8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta

Sample type: 1. HCT116 lysate (50ug)
2. MCF7 lysate (50ug)
3. rCtIP (30ng)
Primary Dilution: 1:1000
Secondary Antibody: anti-Rabbit IR700
Secondary Dilution: 1:5000
Image Submitted by: Nodar Makharashvili
University of Texas, Austin

Publications

Product: Chen, Nievera, Lee et al.: "Cell cycle-dependent complex formation of BRCA1.CtIP.MRN is important for DNA double-strand break repair." in: The Journal of biological chemistry, Vol. 283, Issue 12, pp. 7713-20, 2008 (PubMed).