Centromere Protein B (CENPB) (C-Term) antibody

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Antigen
Synonyms CENPB, CENP-B
Epitope
C-Term
(7), (4), (2), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(30), (13), (13)
Host
Rabbit
(27), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(16), (11), (6), (5), (4), (4), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the C terminal of human CENPB
Blocking Peptide Blocking peptide for this product available: Centromere Protein B (CENPB) peptide (ABIN974834)
Sequence GGEDSDSDSE EEDDEEEDDE DEDDDDDEED GDEVPVPSFG EAMAYFAMVK
Predicted Reactivity Human : 100 %, Mouse : 90 %, Rat : 90 %, Dog : 80 %, Horse : 80 %, Pig : 80 %, Sheep : 80 %
Characteristics This is a rabbit polyclonal antibody against CENPB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CENPB (CENPB Antibody Abstract)
Background CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Molecular Weight 65 kDa
Gene ID 1059
NCBI Accession NP_001801, NM_001810
UniProt P07199
Research Area Chromatin and Nuclear Signaling, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 599 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Centromere Protein B (CENPB) (C-Term) antibody (ABIN503289) WB Suggested Anti-CENPB Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Orthaus, Biskup, Hoffmann et al.: "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells." in: Chembiochem : a European journal of chemical biology, Vol. 9, Issue 1, pp. 77-92, 2007 (PubMed).

Catalog No. ABIN503289
$289.00
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