Pim-1 Oncogene (PIM1) antibody
| Antigen |
|
| Synonyms |
PIM, Pim-1, pim2, cb1068, fj47b05, wu:fj47b05, pim1-a, PIM1, pim3, MGC89574, pim-1, PAP-1 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
|
|
Conjugate
|
|
|
Application
|
|
| Catalog no. |
ABIN503951 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 2 to 3 Business Days |
|
Alternative name
|
PIM1
|
|
Gene ID
|
PIM1
|
|
UniProt
|
P11309-2
|
|
Immunogen
|
The immunogen for anti-PIM1 antibody: synthetic peptide directed towards the N terminal of human PIM1
|
|
Sequence
|
MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQV GPLLGSGGFG
|
|
Cross-Reactivity
|
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
|
|
Format
|
Lyophilized
|
|
Reconstitution
|
Add 50 µl of distilled water. Final anti-PIM1 antibody concentration is 1 mg/ml.
|
|
Description
|
PIM1 may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and
|
|
Characteristics
|
Antigen Size: 313 amino acids
|
|
Molecular Weight
|
36kDa
|
|
Application Notes
|
WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate.
|
|
Purification
|
Affinity Purified
|
|
Buffer
|
PBS
|
|
Storage (Long Term)
|
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Storage (Short Term)
|
Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
|
|
Research Area
|
Cancer, Hematopoietic Progenitors
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Wernig, Gonneville, Crowley et al.: "The Jak2V617F oncogene associated with myeloproliferative diseases requires a functional FERM domain for transformation and for expression of the Myc and Pim proto-oncogenes." in: Blood, Vol. 111, Issue 7, pp. 3751-9, 2008 (PubMed).
|
Alternatives for antigen "Pim-1 Oncogene (PIM1)", type "Antibodies"
|
Hosts
|
Rabbit (32), Mouse (8), Goat (2)
|
|
Reactivities
|
Human (34), Mouse (Murine) (10), Rat (Rattus) (10), Cow (Bovine) (5), Dog (Canine) (5), Pig (Porcine) (5), Sheep (Ovine) (5)
|
|
Applications
|
Western Blotting (WB) (28), ELISA (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunofluorescence (IF) (11), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (2)
|
|
Conjugates
|
Biotin (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), PE,Cy5.5 (1)
|
|
Epitopes
|
C-Term (5), pTyr218 (4), AA 24-37 (2), N-Term (2), Internal Region (1), pTyr118 (1)
|
2013 BioMed Central Research Awards
