PIM1 antibody (C-Term)

Catalog No. ABIN392440

Product Details anti-PIM1 Antibody

Target: PIM1 (Pim-1 Oncogene (PIM1))

Binding Specificity: AA 374-404, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-404 amino acids from the C-terminal region of human PIM1.

Clone: RB1285-RB1286

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIM1 antibody (ABIN392440)

Albertson, Schmidt, Bearss, Tripp, Bearss, Liu: "Patterns and Significance of PIM Kinases in Urothelial Carcinoma." in: Applied immunohistochemistry & molecular morphology : AIMM, Vol. 23, Issue 10, pp. 717-23, (2016) (PubMed).

Plum, Warnecke-Eberz, Dhaouadi, Alakus, Drebber, Metzger, Prenzel, Hölscher, Bollschweiler: "Molecular markers predicting lymph node metastasis in early esophageal cancer." in: Histology and histopathology, pp. 11618, (2015) (PubMed).

Warnecke-Eberz, Bollschweiler, Drebber, Metzger, Baldus, Hölscher, Mönig: "Prognostic impact of protein overexpression of the proto-oncogene PIM-1 in gastric cancer." in: Anticancer research, Vol. 29, Issue 11, pp. 4451-5, (2009) (PubMed).

Chen, Kobayashi, Darmanin, Qiao, Gully, Zhao, Kondo, Wang, Wang, Yeung, Lee: "Hypoxia-mediated up-regulation of Pim-1 contributes to solid tumor formation." in: The American journal of pathology, Vol. 175, Issue 1, pp. 400-11, (2009) (PubMed).

Reiser-Erkan, Erkan, Pan, Bekasi, Giese, Streit, Michalski, Friess, Kleeff: "Hypoxia-inducible proto-oncogene Pim-1 is a prognostic marker in pancreatic ductal adenocarcinoma." in: Cancer biology & therapy, Vol. 7, Issue 9, pp. 1352-9, (2008) (PubMed).

Wallentine, Kim, Seiler, Vaughn, Crockett, Tripp, Elenitoba-Johnson, Lim: "Comprehensive identification of proteins in Hodgkin lymphoma-derived Reed-Sternberg cells by LC-MS/MS." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 11, pp. 1113-24, (2007) (PubMed).

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: PIM1 (PIM1 Products)

Background: PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.

Molecular Weight: 35686

Gene ID: 5292

NCBI Accession: NP_001230115, NP_002639

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process