anti-EEF2 antibody (Eukaryotic Translation Elongation Factor 2) (N-Term)

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Antigen
Synonyms eef2, MGC76191, MGC79628, EEF2, Dmel\CG4849, EFTUD2, eEF2, EEF-2, EF-2, EF2, Ef-2
Alternatives
Epitope
N-Term
(25), (14), (12), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Caenorhabditis elegans (C. elegans), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)
(113), (55), (52), (4), (4), (4), (2), (2), (1), (1)
Host
Rabbit
(97), (16)
Clonality
Polyclonal
Conjugate
This EEF2 antibody is un-conjugated
(5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(80), (46), (39), (37), (24), (23), (14), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human EEF2
Blocking Peptide Blocking peptide for this product available: Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) peptide (ABIN976152)
Sequence TDSLVCKAGI IASARAGETR FTDTRKDEQE RCITIKSTAI SLFYELSEND
Predicted Reactivity African clawed frog : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against EEF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name EEF2 (EEF2 Antibody Abstract)
Background EEF2 is a member of the GTP-binding translation elongation factor family. The protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Molecular Weight 94 kDa
Gene ID 1938
NCBI Accession NP_001952, NM_001961
UniProt P13639
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways AMPK Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 858 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-EEF2 antibody (Eukaryotic Translation Elongation Factor 2) (N-Term) (ABIN504471) WB Suggested Anti-EEF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-EEF2 antibody (Eukaryotic Translation Elongation Factor 2) (N-Term) (ABIN504471) anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) antibody (Image 2)
Background publications Tu, Yan, Hood et al.: "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 4, pp. 575-88, 2007 (PubMed).

Catalog No. ABIN504471
$289.00
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