Cyclin-Dependent Kinase 9 (CDK9) (N-Term) antibody

Details for Product No. ABIN504585
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Antigen
Synonyms CDK9, CG5179, Dmel\\CG5179, P-TEF, P-TEFb, PTefb, cdk9, c-2k, cdc2l4, ctk1, pitalre, tak, C-2k, CDC2L4, CTK1, PITALRE, TAK, wu:fb22e09, wu:fc06c02
Epitope
N-Term
(19), (18), (10), (7), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(100), (45), (41), (26), (26), (26), (14), (8), (2), (1), (1)
Host
Rabbit
(80), (21)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(82), (45), (24), (22), (20), (16), (7), (6), (5), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN504585
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human CDK9
Sequence MAKQYDSVEC PFCDEVSKYE KLAKIGQGTF GEVFKARHRK TGQKVALKKV
Predicted Reactivity Chicken : 100 %, Dog : 100 %, Human : 100 %, African clawed frog : 90 %, Bovine : 90 %, Mouse : 90 %, Pig : 90 %, Rat : 90 %, Zebrafish : 90 %
Characteristics This is a rabbit polyclonal antibody against CDK9. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CDK9
Background CDK9 is the member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Molecular Weight 43 kDa
Gene ID 1025
NCBI Accession NP_001252, NM_001261
UniProt P50750
Research Area Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 372 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Cyclin-Dependent Kinase 9 (CDK9) (N-Term) antibody WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
anti-Cyclin-Dependent Kinase 9 (CDK9) (N-Term) antibody (2) Immunohistochemistry with Uterus tissue at an antibody concentration of 5µg/ml using anti-CDK9 antibody (AVARP03004_P050)
Background publications Sabò, Lusic, Cereseto et al.: "Acetylation of conserved lysines in the catalytic core of cyclin-dependent kinase 9 inhibits kinase activity and regulates transcription." in: Molecular and cellular biology, Vol. 28, Issue 7, pp. 2201-12, 2008 (PubMed).

Validation Images
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