IER5
Reactivity: Human
WB, IF (p), IF (cc)
Host: Rabbit
Polyclonal
AbBy Fluor® 555
Application Notes
Peptide ELISA: Antibody Detection Limit Dilution: 1/2000. Western Blot: 1-3 μg/mL. Customer's experiments gave a band at approx 45 kDa in lysatesof transfected H1299 overexpressing IER5 but not when transfected with empty vector(calculated MW of 33.5 kDa according to NP_057629.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.Synonyms: Immediate early response gene 5 protein, PP4583, SBBI48