TRPM7 antibody (C-Term)

Catalog No. ABIN374098

Product Details anti-TRPM7 Antibody

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPM7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-TKESESTNSV RLML

Specificity: This antibody detects TRPM7 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine (Dog) and Pig.
Species reactivity (tested):Mouse, Rat, Crayfish.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_060142.3. Genename: TRPM7

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx.150 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 210 kDa according to NP_060142.2). The observed molecularweight corresponds to earlier findings in literature with different antibodies. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Alternative Name: TRPM7 (TRPM7 Products)

Synonyms: cb495 antibody, TRPM7 antibody, chak antibody, chak1 antibody, ltrpc7 antibody, trp-plik antibody, ALSPDC antibody, CHAK antibody, CHAK1 antibody, LTRPC7 antibody, TRP-PLIK antibody, 2310022G15Rik antibody, 4833414K03Rik antibody, 5033407O22Rik antibody, LTrpC-7 antibody, Ltpr7 antibody, Ltrpc7 antibody, TRPPLIK antibody, Chak antibody, Trp-plik antibody, transient receptor potential cation channel subfamily M member 7 antibody, transient receptor potential cation channel, subfamily M, member 7 antibody, TRPM7 antibody, trpm7 antibody, Trpm7 antibody

Background: The TRPM7 (Transient Receptor Protein-PhosphoLipase C-Interacting Kinase) protein is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. TRPM7, however, is unique among the TRP channel proteins because it is bi-functional. It not only controls ion channels but it also acts as a kinase, phosphorylating proteins.Synonyms: CHAK1, Channel-kinase 1, LTRPC7, Long transient receptor potential channel 7, Transient receptor potential cation channel subfamily M member 7

Gene ID: 54822

NCBI Accession: NP_060142

UniProt: Q96QT4