Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 35 kDa band seen in HeLa cell lysates. Please note theband size is in agreement with previous experimental data (J Biol Chem 2001 Apr27,276(17): 13718-26). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Testis shows staining ofselect spermatocytes in Human Testis. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.Synonyms: ATF-7, ATF-A, ATFA, Activating transcription factor 7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7