GPX4 antibody (C-Term, Isoform A)
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- Target See all GPX4 Antibodies
- GPX4 (Glutathione Peroxidase 4 (GPX4))
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Binding Specificity
- C-Term, Isoform A
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Reactivity
- Human, Mouse, Rat, Cow, Pig
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GPX4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1).
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Rat, Pig, Cow.
Species reactivity (tested):Human. - Purification
- Affinity Chromatpography
- Immunogen
- Peptide with sequence (C-EEPLVIEKDLPHY) from the C-terminus of the protein sequencen (NP_002076.2, NP_001034937.1).
- Top Product
- Discover our top product GPX4 Primary Antibody
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- Application Notes
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ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- GPX4 (Glutathione Peroxidase 4 (GPX4))
- Alternative Name
- Glutathione Peroxidase 4 / GPX4 (GPX4 Products)
- Synonyms
- GPx-4 antibody, GSHPx-4 antibody, MCSP antibody, PHGPx antibody, snGPx antibody, snPHGPx antibody, Phgpx antibody, gpx-4 antibody, snGpx antibody, cb563 antibody, cb691 antibody, PHGPxa antibody, sb:cb563 antibody, zgc:92253 antibody, wu:fb82e03 antibody, 1700027O09Rik antibody, mtPHGPx antibody, ATGPX4 antibody, F11L15.5 antibody, glutathione peroxidase 4 antibody, GP-GPx4 antibody, glutathione peroxidase 4 antibody, glutathione peroxidase 4a antibody, GPX4 antibody, Gpx4 antibody, gpx4a antibody
- Background
- Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial
- Gene ID
- 2879
- NCBI Accession
- NP_001034936
- UniProt
- P36969
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