Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This Antithrombin III antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of Human Antithrombin III.
ELISA: 1/1,000Western blotting: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C or (in aliquots) at -20 °C for longer.
AT3 antibody, AT3D antibody, ATIII antibody, THPH7 antibody, SERPINC1 antibody, DKFZp470C1733 antibody, AI114908 antibody, At-3 antibody, At3 antibody, ANTITHROMBIN, AT-III antibody, AT-III antibody, serpin family C member 1 antibody, antithrombin-III antibody, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 antibody, SERPINC1 antibody, CpipJ_CPIJ000472 antibody, CpipJ_CPIJ013111 antibody, Serpinc1 antibody
Background
Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160 mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58 kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties.Synonyms: AT3, ATIII, Serpin C1