CAS9 antibody

Details for Product No. ABIN5563965, Supplier: Log in to see
Antigen
Reactivity
Streptococcus pyogenes (S. pyogenes)
8
1
Host
Mouse
5
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
9
4
3
3
3
2
Options
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Clone 8C1-F10
Isotype IgG2b
Specificity Mouse
Purification Protein A Chromatography
Background Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Molecular Weight 160 kDa
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-CAS9 antibody (ABIN5563965) Cas9 antibody (mAb) tested by ChIP. NIH3T3 cells stably expressing GFP-H2B, nuclease ...
Immunofluorescence (IF) image for anti-CAS9 antibody (ABIN5563965) Cas9 antibody (mAb) tested by Immunofluorescence. Hela cells or Hela cells expressing...
Western Blotting (WB) image for anti-CAS9 antibody (ABIN5563965) Cas9 antibody (mAb) tested by Western blot. Hela cells and Hela cells expressing Flag...
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