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CRISPR-Cas9 (C-Term) antibody (DyLight 549)

Reactivity: Streptococcus pyogenes WB, ELISA, FM Host: Rabbit Polyclonal DyLight 549
Catalog No. ABIN6658288
  • Target
    CRISPR-Cas9
    Binding Specificity
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    Streptococcus pyogenes
    Host
    • 15
    • 10
    Rabbit
    Clonality
    • 13
    • 12
    Polyclonal
    Conjugate
    • 18
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    DyLight 549
    Application
    • 23
    • 9
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Purification
    Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Immunogen

    Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

    Immunogen Type: Peptide

    Isotype
    IgG
    Labeling Ratio
    4.8
  • Application Notes

    Application Note: Cas9 DyLight™549 Conjugated Antibody has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

    ELISA Dilution: 1,25,000-1:30,000

    Western Blot Dilution: 1 μg/mL

    IF Microscopy Dilution: 1 μg/mL

    Other Performance Data: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    CRISPR-Cas9
    Alternative Name
    Cas 9
    Background

    Synonyms: rabbit anti-Cas9 antibody DyLight™ 549 conjugation, DyLight™ 549 conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

    Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

    Gene Name: cas9

    Gene ID
    901176
    NCBI Accession
    NP_269215
    UniProt
    Q99ZW2
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