»
Antibodies
»
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
| Antigen |
|
| Synonyms |
ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940 |
| Clonality |
Monoclonal (E32) |
|
Host
|
|
|
Reactivity
|
Human (139), Rat (Rattus) (65), Mouse (Murine) (62), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
|
|
Application
|
 Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (34), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
|
| Catalog no. |
ABIN559693 |
| Quantity |
100ug (1mg/mL) |
| Price |
269.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 to 7 Business Days |
|
Alternative name
|
ERK1
|
|
Immunogen
|
Recombinant full length ERK1 protein
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
E32
|
|
Specificity
|
Recognizes only the ERK1 protein (e.g.: from human and mouse cells). WB: ERK1 from human and mouse cells / IP: ERK1 from human cells / ERK1 from other species may also be detectable
|
|
Application Notes
|
WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test
|
|
Concentration
|
1mg/mL
|
|
Purification
|
Protein A affinity chromatography from mouse ascites fluid
|
|
Buffer
|
10mM PBS, pH 7.2, 0.05% NaN3
|
|
Storage
|
Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
|
Hosts
|
Rabbit (114), Mouse (29), Goat (6), Chicken (1), Sheep (1)
|
|
Reactivities
|
Human (139), Rat (Rattus) (65), Mouse (Murine) (62), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
|
|
Applications
|
Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (34), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (24), Immunoprecipitation (IP) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
|
|
Conjugates
|
Biotin (1), HRP (1)
|
|
Epitopes
|
pTyr204 (8), N-Term (7), pThr202 (7), pThr202/pTyr204 (5), C-Term (4), pThr202,pTyr204 (2), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT-E-pY Motif (1), pT202 (1), pY204 (1)
|
